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© 1972 Oxford University Press

RESEARCH-ARTICLE

Protein Changes in the Vascular Wilt Disease of Lucerne caused by Verticillium albo-atrum R. et B

P. J. WHITNEY 1, J. B. HEALE and J. G. VAUGHAN

Department of Biology, Queen Elizabeth College Campden Hill, London, W. 8

Field trials confirmed that, whereas the Du Puits variety of lucerne was highly susceptible to Verticillium albo-atrum, ‘Gaetula’ hybrids showed slight resistance and certain ‘Swedish’ material was moderately resistant. Symptom-free ‘Gaetula’ and Swedish plants were used to produce uninfected clonal material by root propagation.

Comparisons of the proteins of such plants with seed-grown Du Puits produced no evidence of a correlation between protein pattern before inoculation and differences in susceptibility and resistance.

Disc electrophoretic comparisons of infected host material showed that the pathogen gradually induced a change in the shoot proteins of Du Puits whereas in the resistant host, after an initial marked change, there was a development towards normality. By the terminal stages of wilting there was a noticeable fall in the numbers of proteins in both roots and shoots of Du Puits, a finding confirmed both by double diffusion and immunoelectrophoresis. At the same time there was good evidence for a slight increase in protein bands in the resistant material after inoculation.

After feeding tritium-labelled leucine to infected Du Puits plants, a small protein component of fungal origin was detected in the host. Labelled components from both healthy and infected Du Puits plants were separated on Sephadex G.25 and compared.


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