Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by COCKBURN, W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by COCKBURN, W.
Agricola
Right arrow Articles by COCKBURN, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1974 Oxford University Press

RESEARCH-ARTICLE

Simultaneous Measurement of Oxygen Evolution and Endogenous Adenosine-Triphosphate Levels in Isolated ‘Intact’ Chloroplast Suspensions

W. COCKBURN

Botanical Laboratories, University of Leicester Leicester, LE1 7RH

Simultaneous continuous polarographic estimation of oxygen evolution and measurement of ATP levels by the firefly luciferase method were made in suspensions containing predominantly intact spinach chloroplasts. Measurements were made during light to dark and dark to light transitions in the presence and absence of bicarbonate, 3-phosphoglycerate and ribose-5-phosphate and following the addition of these compounds during steady state conditions.

Absolute levels of ATP were usually in the range 3.7 µmol ATP per g chlorophyll and the results of kinetic experiments are interpreted within the established concepts of photophosphorylation and the carbon reduction cycle.

In experiments involving dark to light transitions in the absence of added bicarbonate, light to dark transitions in the presence of 3-phosphoglycerate and during photosynthesis in the presence of bicarbonate, 3-phosphoglycerate and ribose-5-phosphate rapid transient changes in ATP levels were observed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.