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© 1974 Oxford University Press

RESEARCH-ARTICLE

Starch Metabolism in Shoot-forming Tobacco Callus

TREVOR A. THORPE and DONALD D. MEIER

Department of Biology, University of Calgary Calgary, Alberta, Canada, T2N 1N4

Starch, free sugars and protein contents, and the specific activities of enzymes of starch metabolism were determined in tobacco callus cultured under shoot-forming and non-shoot-forming conditions. Shoot-forming cultures contained higher levels of starch, free sugars and protein. Shoot-forming cultures had higher specific activities for starch-synthesising enzymes throughout culture. On the other hand, higher levels of activity for starch-degrading enzymes in shoot-forming tissues were only observed during organized development. The role of phosphorylase in the cultured tissue was not clear.


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