Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by GOULD, A. R.
Right arrow Articles by STREET, H. E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by GOULD, A. R.
Right arrow Articles by STREET, H. E.
Agricola
Right arrow Articles by GOULD, A. R.
Right arrow Articles by STREET, H. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1974 Oxford University Press

RESEARCH-ARTICLE

Studies on the Growth in Culture of Plant Cells

XVII. ANALYSIS OF THE CELL CYCLE OF ASYNCHRONOUSLY DIVIDING ACER PSEUDOPLATANVS L. CELLS IN SUSPENSION CULTURE

A. R. GOULD, M. W. BAYLISS and H. E. STREET

Botanical Laboratories, School of Biological Sciences, University of Leicester Leicester, LEI 7RH

Three methods of cell cycle analysis, involving the use of tritiated thymidine, have been applied to asynchronously dividing suspension cultures of sycamore. Conditions for an effective chase of unlabelled thymidine were established from a study of the kinetics of entry and incorporation of tritiated thymidine into the cells. The levels of thymidine used did not affect the rate of cell division or the duration of the phases of the cell cycle.

The analyses of the cell cycle based upon pulse labelling, continuous labelling, and a combination of densitometry and autoradiography were in good agreement and showed that the phases S (mean 7.0 h), G2 (mean 8.5 h) and mitosis (mean 3.0 h), were of relatively constant duration, whereas G1 was of variable duration.

No relation between nuclear DNA content and mitotic-cycle time or the duration of S-phase could be inferred from the data presented.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.