Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by DICK, P. S.
Right arrow Articles by REES, T. A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by DICK, P. S.
Right arrow Articles by REES, T. A.
Agricola
Right arrow Articles by DICK, P. S.
Right arrow Articles by REES, T. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1975 Oxford University Press

RESEARCH-ARTICLE

Effects of Water Stress on the Respiratory and Nitrogen-fixing Activity of Soybean Root Nodules

P. S. DICK and T. AP REES

Botany School, University of Cambridge Cambridge, Cambridge CB2 3EA

The aim of this work was to discover whether sugar moved into the cortex and apex of roots of Pisum sativum via the symplasm or the free space. The method was to observe whether flooding the free space of the root with sugar solutions inhibited the movement of 14C from the stele into the surrounding cells. Seedlings were arranged in a compartmented box so that the free space of the apical 23 mm of the roots could be flooded with sugar, and [14C]glucose could be applied locally to regions of the steles, 36–46 mm from the apices, that had been stripped of cortex. After incubation, the apical 6 mm of the roots were excised, and the regions 6–23 mm from the apex were separated into stele and cortex. The movement of 14C into this cortex and apex was not reduced by flooding the free space with any of the following: 1.5 mM, 50 mM sucrose; 0.3 mM, 3.0 mM, 100 mM glucose; 3.0 mM fructose. When [14C]glucosyl sucrose was applied to the steles, the asymmetrical labelling was retained in the sucrose isolated from the apical 6 mm and the cortex. In comparable experiments seedlings were allowed to photosynthesize in 14CO2- The presence of 1.5 mM sucrose or 3.0 mM glucose in the free space of the apical 46 mm of the roots of these seedlings did not reduce the movement of label into the cortex and apex. These results are held to be consistent with the symplasmic transport of sugar from the stele into the surrounding cells.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
F. Ma and C. A. Peterson
Frequencies of plasmodesmata in Allium cepa L. roots: implications for solute transport pathways
J. Exp. Bot., May 1, 2001; 52(358): 1051 - 1061.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
R. M. Gifford, J. H. Thorne, W. D. Hitz, and R. T. Giaquinta
Crop Productivity and Photoassimilate Partitioning
Science, August 24, 1984; 225(4664): 801 - 808.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.