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© 1987 Oxford University Press

RESEARCH-ARTICLE

Cold Acclimation, Freezing Resistance and Protein Synthesis in Alfalfa (Medicago sativaL. cv. Saranac)

SHYAM S. MOHAPATRA, RONALD J. POOLE and RAJINDER S. DHINDSA

Centre for Plant Molecular Biology, Department of Biology, McGill University Montreal, Quebec H3A 1B1, Canada

To whom correspondence should be addressed.

Mohapatra, S. S., Poole, R. J. and Dhindsa, R. S. 1987. Cold acclimation, freezing resistance and protein synthesis in alfalfa (Medicago sativa L. cv. Saranac).—J. exp. Bot. 38: 1697–1703.

Changes in freezing resistance (percent survival at —10 °C), pattern of protein synthesis and translatable mRNA population during cold acclimation of alfalfa (Medicago sativa L. cv. Saranac) have been examined. Two days of cold acclimation at 4 °C increased freezing resistance from about 6% to 40%, protein content by 200% and total RNA content by 100%. Acclimation for longer periods did not cause further increases in freezing resistance, protein content or RNA content. Examination of protein changes by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) coupled with protein staining, and by fluorography of in vivo labelled proteins separated by SDS-PAGE, showed that several proteins are increasingly or newly synthesized during cold acclimation. Analysis of in vitro translation products by SDS-PAGE and fluorography shows changes in the population of translatable mRNAs. It is concluded that in this variety of alfalfa cold acclimation for only 2 d is sufficient to confer maximum freezing resistance, and that changes in proteins during cold acclimation are regulated most probably at the transcnptional level.

Key words: Freezing resistance, protein synthesis, cold acclimation, SDS-PAGE, Medicago sativa L.


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