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© 1987 Oxford University Press

RESEARCH-ARTICLE

Development of Epidermal Cell Wall Peroxidases along the Mung Bean Hypocotyl: Possible Involvement in the Cell Wall Stiffening Process

R. GOLDBERG, M. LIBERMAN, C. MATHIEU, M. PIERRON and A. M. CATESSON

Université Pierre et Marie Curie et Ecole Normale Supérieure, Laboratoire des Biomembranes et Surfaces Cellulaires Végétales U.A. 04 311, 46 rue d'Ulm, 75230 Paris Cedex 05, France

Correspondence to: Université Pierre et Marie Curie et Ecole Normale Superieure, Laboratoire des Biomembranes et Surfaces Cellulaires Vegetales, U.A. 04 311, 46 rue d'Ulm, 75230 Paris Cedex 05, France.

Goldberg, R., Liberman, M., Mathieu, C, Pierron, M. and Catesson, A. M. 1987. Development of epidermal cell wall peroxidases along the mung bean hypocotyl: possible involvement in the cell wall stiffening process.—J. exp. Bot. 38: 1378–1390.

Ultrastructural investigation showed that in the epidermis of mung bean hypocotyls, cell wall peroxidatic activities could be detected mainly below the maximal elongation zone. In the epidermis the peroxidatic activities were preferentially located in the radial cell walls. Cell wall peroxidases were then isolated from epidermal strips and further characterized. The possible presence of a H2O2-generating system in the epidermis of mung bean hypocotyls was also investigated. When whole segments were processed for electron microscopy, H2O2 could be detected cytochemically in the cell walls with the CeCl3 technique. A positive reaction was obtained in the same location when specimens were incubated in a 3-3'-diaminobenzidine medium for peroxidases in which H2O2 was replaced by its possible precursors (NADH or NAD + malate). However, isolated epidermal cell walls could not generate H2O2 at the expense of NADH although they were able to oxidize the reduced nicotinamide-adenine-dinucleotide. The possible relationships between peroxidase activities, H2O2, and Ca2+ ions are discussed with respect to their involvement in the cell wall stiffening process.

Key words: Epidermis, cell wall, elongation, peroxidases


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