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© 1987 Oxford University Press

RESEARCH-ARTICLE

Growth Regulation of Galium mollugo L. Cell Suspensions by a-Naphthalene Acetic Acid

CATHERINE FIDGEON and GRAHAM WILSON

To whom correspondence should be addressed.

Fidgeon, C. and Wilson, G. 1987. Growth regulation of Galium mollugo L. cell suspensions by {alpha}-naphthalene acetic acid.—J. exp. Bot. 38: 1491–1500.

Galium mollugo cell suspension cultures were found to require the plant growth regulator {alpha}-naphthalene acetic acid ({alpha}-NAA) for continued growth and cell division. This requirement could not be substituted in either batch or semi-continuous culture by indole-3-acetic acid (IAA) or 2,4-dichlorophenoxy acetic acid (2,4-D) at any concentration tested. However, ß-naphthalene acetic acid (ß-NAA) and indole-3-butyric acid (IBA) were found to support growth when supplied at a concentration two orders of magnitude greater than the normal media level (0–5 mg dm3).

The growth of Galium cells was found to be influenced not only by the {alpha}-NAA initially supplied in the medium but also by the exposure to {alpha}-NAA in previous growth cycles. Preculture of cells for 3 d in an {alpha}-NAA containing medium, followed by cell washing and re-inoculation into {alpha}-NAA free medium, supported a quantitative growth response similar to that obtained after 14 d in the control {alpha}-NAA containing medium. Even short-term exposures between 0·5 and 6·0 h stimulated a detectable growth response 14 d later. These observations raise questions relating to the uptake and perception of exogenously supplied growth regulators by cultured cells.

The delayed kinetics of this form of response is of significance in culture regimes in which cells are transferred from one medium to another, differing in their growth regulator composition, in order to induce morphogenesis


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