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© 1987 Oxford University Press

RESEARCH-ARTICLE

Transformation of Solarium and Nicotiana Species using an Ri Plasmid Vector

M. R. DAVEY1, B. J. MULLIGAN1, K. M. A. GARTLAND, E. PEEL2, A. W. SARGENT2 and A. J. MORGAN2

1Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park Nottingham NG7 2RD, U.K.
2B.P. Research Centre, Chertsey Road, Sunbury-on-Thames Middlesex TW16 7LN, U.K.

Present address: School of Life Sciences, Leicester Polytechnic, Scraptoft, Leicester LE7 9SU, U.K.

Davey, M. R., Mulligan, B. J., Gartland, K. M. A., Peel, E., Sargent, A. W. and Morgan, A. J. 1987. Transformation of Solanum and Nicotiana species using an Ri plasmid vector.—J. exp. Bot. 38: 1507–1516.

Five Nicotiana species (N. benthemiana, N. debneyi, N. occidentals, N. plumbaginifolia, N. tabacum) and three Solanum species (S. dulcamara, S. nigrum, S. tuberosum) were transformed by wild-type and engineered Ri plasmids. Depending on the host plant, roots transformed by Agrobacterium strain A4TIII with an Ri plasmid carrying a chimaeric nopaline synthase-kanamycin resistance gene, were 3 to 40 times more resistant to kanamycin than roots transformed by the wild-type plasmid of strain A4T. Similarly, plants regenerated from A4TIII-derived roots of N. debneyi, N. plumbaginifolia and N. tabacum were 8 to 16 times more resistant than A4T plants, and survived at 400 µg cm3 of kanamycin. A4TIII plants of S. nigrum flowered in vitro at 600–1000 µg cm3 of kanamycin. Transformed roots and most regenerated plants synthesized Ri-speciflc opines, while DNA hybridization confirmed the presence of DNA homologous to that from wild-type and engineered Ri plasmids in transformed plants of S. nigrum.

Key words: Agrobacterium, Ri plasmid, transformed roots, plant regeneration, kanamycin resistance.


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