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© 1989 Oxford University Press

RESEARCH-ARTICLE

In Vitro Shoot Regeneration from Callus, Leaf Axils and Petioles of Sugar Beet (Beta vulgaris L.)

G. A. RITCHIE, K. C. SHORT and M. R. DAVEY1

School of Science, Trent Polytechnic New Science Building, Clifton, Nottingham NG11 8NS, UK
1 Plant Genetic Manipulation Group, Department of Botany, University of Nottingham Nottingham NG7 2RD, UK

Procedures are described for producing axillary shoots from seedling apices and adventitious shoots from petioles and leaf-derived callus of sugar beet cultivars. The rate of adventitious shoot regeneration from petioles was influenced by temperature, BAP concentration of the medium, and the time in culture of the seedling apices from which the petioles were excised. Petiole sectioning confirmed that adventitious shoots originated in the sub-epidermal parenchyma. Two distinct types of callus were produced from leaf explants, but only white friable callus was capable of shoot development. This callus developed from brown tissue and was composed of thin-walled cells with dense cytoplasm and prominent nuclei. Green compact callus with thick-walled lignified cells developed from green tissue, but did not produce shoots. Successful seed sterilization and shoot regeneration from petiole explants and callus was cultivar-dependent. Adventitious shoots were rooted and successfully transplanted to potting compost under glasshouse conditions.

Key words: Adventitious shoots, axillary shoots, callus, sugar beet (Beta vulgaris L.)


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