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© 1992 Oxford University Press

RESEARCH-ARTICLE

High Efficiency Plant Regeneration from Protoplasts of the Indica Cultivar IR58

L. B. TORRIZO and F J. ZAPATA

Tissue 12 P.O. Box 933, Manila, Philippines

To correspondence should be addressed

Procedures have been established for embryogenic cell suspension, protoplast culture, and plant regeneration from the Indica rice cultivar, IR58. Embryogenic cell suspension was established in modified R2 medium and maintained in amino acid-based AA medium. Protoplasts were cultured in R2 basal medium containing B5 vitamins. Plants were regenerated in MS basal medium with 3% maltose as carbon source. Selection of embryogenic protoplast-derived calli is an important step for high-frequency plant regeneration. More than 900 plants were regenerated through this procedure.

Key words: Rice (Oryza sativa, Indica, Protoplast, Maltose


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