Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by BESSE, I.
Right arrow Articles by MIGINIAC, E
Right arrow Search for Related Content
PubMed
Right arrow Articles by BESSE, I.
Right arrow Articles by MIGINIAC, E
Agricola
Right arrow Articles by BESSE, I.
Right arrow Articles by MIGINIAC, E
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1992 Oxford University Press

RESEARCH-ARTICLE

Oil Palm (Elaeis guineensis Jacq.) Clonal Fidelity: Endogenous Cytokinins and Indoleacetic Acid in Embryogenic CallusCultures

I. BESSE1,2,3, J L. VERDEIL1, Y DUVAL1, B. SOTTA2, R. MALDINEY2 and E MIGINIAC2

1LRGAPT, ORSTOM-IRHO/CIRAD, BP 5045, Montpellier cedex O1, France
2Laboratoire de Physiologie du Dvéloppement des Plantes, URA, CNRS 1180, Université Paris VI, 4, place Jussieu, 75252, Pariscedex 05, France

3To whom correspondence should be addressed.

Endogenous levels of indole-3-acetic acid (I AA) and cytokinins were measured in the two types of callus of oil palm (Elaeisguineensis Jacq.) by an HPLC-ELISA methodology. The Nodular Compact Callus (NCC) is commonly used to establish stableembryoid strains ensuring clonal fidelity(≤5% abnormal plants) while the soft and friable callus, called Fast Growing Callus(FGC), leads to the malformation of inflorescences in regenerated plants (100% abnormal plants). The most striking result wasthat no significant amounts of cytokinins were detected in FGC whereas they accumulated in NCC. These trends werereproducible during a subculture time. By contrast, IAA levels were not significantly different between each type of callus of thefour clones tested. Endogenous cytokinin levels can be considered as markers of the physiological status of the callus, whateverthe clone. There appears to be a close relationship between the ability of FGC to regenerate plants with abnormal inflorescencesand low levels of cytokinins. The different mechanisms leading to the formation of FGC are discussed.

Key words: Oil palm, cytokinins, IAA, somatic embryogenesis, clonal fidelity


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
A. Rival, E. Jaligot, T. Beule, and E. J. Finnegan
Isolation and expression analysis of genes encoding MET, CMT, and DRM methyltransferases in oil palm (Elaeis guineensis Jacq.) in relation to the 'mantled' somaclonal variation
J. Exp. Bot., September 1, 2008; 59(12): 3271 - 3281.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.