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© 1992 Oxford University Press

RESEARCH-ARTICLE

Unidirectional Ca2+ Fluxes in Roots of Rye (Secale cereale L). A Comparison of Excised Roots with Roots of Intact Plants

PHILIP J. WHITE, JOHN BANFIELD and MIRIAM DIAZ 2

Department of Botany, University of Cambridge Downing Street, Cambridge CB2 3EA, UK

Present address and to whom correspondence should be sent: Department of Plant Physiology, Horticulture Research International East Mailing, West Mailing, Kent ME19 6BJ, UK.

The unidirectional Ca2+ fluxes across the plasma membrane and tonoplast were determined in both excised roots and roots of intact seedlings of rye (Secale cereale L. cv. Rheidol). The unidirectional Ca2+ fluxes across the plasma membrane and tonoplast measured in excised roots were of a similar order of magnitude to those determined in roots of intact plants. Influx and efflux of Ca2+ across the root plasma membrane were similar (estimated to be between 0·7 and 3·4 µmol gminusb1 FW root hminusb1) and 4- to 40- fold greater than the net uptake of calcium into roots (76 nmol gminusb1 FW hminusb1 in excised roots and 427 nmol gminusb1 FW hminusb1 in roots of intact plants). The unidirectional Ca2+ fluxes across the tonoplast (estimated to be between 20 and 100 nmol gminusb1 FW hminusb1) were at least an order of magnitude lower than those across the plasma membrane. In intact plants, the root vacuole accumulated calcium during the experiment at a rate of 30 nmol gminusb1 FW hminusb1, whereas a net loss of calcium from the vacuole (31 nmol gminusb1 FW hminusb1) was calculated for excised roots. The Ca2+ flux to the xylem was greater in intact plants (215 to 393 nmol g-1 FW hminusb1) than in excised roots (107 nmol gminusb1 FW hminusb1), which largely accounted for the differences in calcium uptake by roots from the two treatments.

Key words: Calcium, Ca2+, influx, Ca2+, efflux, rye (Secale cereale L. cv. Rheidol)


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