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© 1992 Oxford University Press

RESEARCH-ARTICLE

A Topographic Method for Studying Uptake, Translocation and Distribution of Inorganic Ions using Two Radiotracers Simultaneously

RICHARD TYKVA1,, IVANA MACHACKOVA2, JAN KREKULE2 and RUDOLF JISL1

1Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences CS-166 10 Praha 6, Czechoslovakia
2Institute of Experimental Botany, Czechoslovak Academy of Sciences CS-160 00 Praha 6, Czechoslovakia

To whom Correspondence should be addressed

A novel topographic method is described for the fully automated quantitative resolution of activity of two simultaneously applied beta tracers in plant tissues. The advantages of this method using specially developed silicon detectors are demonstrated in studying the effect of direct current on the uptake and distribution of Ca2+ and P1 ions in leaves and stems of Chenopodium rubrum. This treatment has recently been shown to inhibit flower induction in this plant.

Key words: Radiotracer multi-labelling, computer-controlled resolution, quantitative topographic evaluation


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