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© 1995 Oxford University Press

RESEARCH-ARTICLE

Polypeptides associated with the induction of direct somatic embryogenesis in Camellia japonica leaves I. Identification of embryo-specific polypeptides

M. Cristina Pedroso1,3, Jean-Louis Hilbert2, Jacques Vasseur2 and M. Salomé Pais1

1Departamento de Biologia Vegetal, Faculdade de Ciências, Universidade de Lisboa Bloco C2, Piso 1, Campo Grande, P-1700 Lisboa, Portugal
2Laboratoire de Physiologie Cellulaire et de Morphogenèse Végétales, Université des Sciences et Technologies de Lille F-59655 Villeneuve d'Ascq, France

3To whom correspondence should be addressed. Fax: + 351 1 75 000 48.

Embryogenic and non-embryogenic induced leaves of Camellia japonica, with the same shoot origin and submitted to the same culture conditions, were used to study protein changes during the induction of direct embryogenesis. The analysis of protein changes in these samples, based on two-dimensional polyacrylamide gel electrophoresis, revealed that 91% and 66.2% of the polypeptides detected, respectively, in embryogenic and non-embryogenic induced leaves, were already present in the non-induced control leaves. The results of the differential expression of eight selected polypeptides detected in induced and non-induced leaves are presented. A spotlist report was obtained for the 9% (43) embryogenesis-associated leaf polypeptides. From these polypeptides, two polypeptides were identified which seem to be specifically associated with somatic embryogenesis in C. japonica: E1 (pl 5.6; mol. wt. 43.5) and E2 (pl 6.0; mol. wt. 25.7).

Key words: Camellia japonica, embryogenesis-associated polypeptides, somatic embryogenesis, two-dimensional polyacrylamide gel electrophoresis


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