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© 1995 Oxford University Press

RESEARCH-ARTICLE

Transient gene expression in shoot apical meristems of sugarbeet seedlings after particle bombardment

Andreas Mann1,3, Anja Matzk1, Christof Sautter2 and Joachim Schiemann1

1Institute for Biochemistry and Plant Virology, Federal Biological Research Centre for Agriculture and Forestry Messeweg 11/12, D-38104 Braunschweig, Germany
2Institute of Plant Sciences, Swiss Federal Institute of Technology, Universitatsstr 2, CH-8092 Zurich, Switzerland

3To whom correspondence should be addressed at: Institute for Breeding Methodology in Vegetables, Federal Centre for Breeding Research on Cultivated Plants, Neuer Weg 22/23, D-06484 Quedlinburg, Germany.

Sugarbeet apices were used as targets for particle bombardment with a microtargeting device. Before examining gene expression, particle penetration experiments were carried out. Transient GUS expression was detected within the first and second cell layers of the meristem. Dividing cells with GUS activity demonstrated that cells survived the bombardment procedure.

Key words: Beta vulgaris, direct gene transfer, microtargeting, particle bombardment, shoot apical meristem


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