Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Shivanna, K.R.
Right arrow Articles by Sawhney, V.K.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Shivanna, K.R.
Right arrow Articles by Sawhney, V.K.
Agricola
Right arrow Articles by Shivanna, K.R.
Right arrow Articles by Sawhney, V.K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1995 Oxford University Press

OTHER

Polyethylene glycol improves the in vitro growth of Brassica pollen tubes without loss in germination

K.R. Shivanna1 and V.K. Sawhney2,3

1Department of Botany, University of Delhi Delhi 110007, India
2Department of Biology, University of Saskatchewan 112 Science Place, Saskatoon, SK. S7N 5E2, Canada

3 To whom correspondence should be addressed. Fax: +1 306966 4461

The culture media for the in vitro pollen assay of Brassica species have so far shown good percentage germination, but limited pollen tube growth. It is reported here that by lowering sucrose concentration from 20% to 5 or 10 % and by adding polyethylene glycol 4000 in the culture medium, high percentage germination and high tube growth (10 times that in the standard media) can be achieved in three species of cultivated Brassica. This improved medium should be useful in fundamental and applied studies on the pollen biology of Brassica species.

Key words: Brassica, pollen, pollen germination, pollen tube growth, polyethylene glycol


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
I. S. Sheoran, A. R. S. Ross, D. J. H. Olson, and V. K. Sawhney
Proteomic analysis of tomato (Solanum lycopersicum, formerly hycopersicon esculentum) pollen
J. Exp. Bot., October 5, 2007; (2007) erm199v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
I. Barinova, M. Zhexembekova, E. Barsova, S. Lukyanov, E. Heberle-Bors, and A. Touraev
Antirrhinum majus microspore maturation and transient transformation in vitro
J. Exp. Bot., May 1, 2002; 53(371): 1119 - 1129.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.