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© 1995 Oxford University Press

OTHER

Effect of carbenicillin and cefotaxime on somatic embryogenesis of Sitka spruce (Picea sitchensis (Bong.) Carr.)

K.S. Sarma1, Nicola E. Evans2,3 and Chris Selby1,2

1 Department of Applied Plant Science, The Queen's University of Belfast Newforge Lane, Belfast BT95PX, UK
2 Department of Agriculture for Northern Ireland Newforge Lane, Belfast BT95PX, UK

3Present address and to whom correspondence should be sent The Department of Applied Plant Science, The Queen's University of Belfast Newforge Lane, Belfast BT9 5PX, UK. Fax: +44 1232 668372.

Carbenicillin prevented the development of mature somatic embryos, reduced early stage embryos by >90% and tissue growth by 50%. Cefotaxime had no effect on overall tissue growth, but reduced the development of early and mature embryos by 20% and 66–80%, respectively. Carbenicillin should not be used for transformation of Sitka spruce.

Key words: Sitka spruce, somatic embryogenesis, antibiotic sensitivity


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