© 1995 Oxford University Press
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Ultrastructural localization of the major biotinylated protein from Pisum sativum seeds
Unité Mixte CNRS/RhdnePoulenc, RhdnePoulenc Agrochimie 1420 rue Pierre Baizet, 69263 Lyon cedex 9, France
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SBP65, the major biotinylated protein in mature pea seeds, is devoid of biotin-dependent carboxylase activity. An ultrastructural investigation using an immunocytochemical technique revealed that this protein is localized mainly in the cytosol, i.e. at the site where biotin is synthesized and deposited in the plant cell. The possibility that SBP65 functions as a sink for free biotin during seed development is discussed.
Key words: Biotin, LEA (Late-Embryogenesis Abundant), proteins, seed development
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