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© 1995 Oxford University Press

OTHER

Ultrastructural localization of the major biotinylated protein from Pisum sativum seeds

Manuel Duval, Régis Pépin, Claudette Job, Christine Derpierre, Derpierre Roland and Dominique Job1

Unité Mixte CNRS/Rhdne–Poulenc, Rhdne–Poulenc Agrochimie 14–20 rue Pierre Baizet, 69263 Lyon cedex 9, France

1To whom correspondence should be addressed. Fax: +33 72 29 22 97

SBP65, the major biotinylated protein in mature pea seeds, is devoid of biotin-dependent carboxylase activity. An ultrastructural investigation using an immunocytochemical technique revealed that this protein is localized mainly in the cytosol, i.e. at the site where biotin is synthesized and deposited in the plant cell. The possibility that SBP65 functions as a sink for free biotin during seed development is discussed.

Key words: Biotin, LEA (Late-Embryogenesis Abundant), proteins, seed development


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