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Journal of Experimental Botany, Vol 49, 21-25, Copyright © 1998 by Oxford University Press


ARTICLES

Identification of new minisatellites loci in Arabidopsis thaliana

S Tourmente, A Lazreg, J Lafleuriel, S Tutois, C Cuvillier, M Espagnol and G Picard
UMR 6547 Biomove, Universite Blaise Pascal, 24 Avenue des Landais, F-63177 Aubiere Cedex, France; Corresponding author e-mail: tourmente@cicsum.univ-bpclemontfr

In order to characterize new CG-rich minisatellites present in the Arabidopsis thaliana genome, a genomic library was screened at low stringency with a probe containing nine repeated-units of a minisatellite (CMs1) previously identified. Both minisatellites and minisatellite-like elements were identified. The minisatellites, with a tandemly-repeated structure, all contain the Arabidopsis thaliana-core sequence previously defined (Tourmente et al., 1994). Both minisatellite and minisatellite-like sequences occur in the Arabidopsis genome in low copy and are weakly polymorphic between ecotypes. The genetic mapping of these markers has shown that they are dispersed on the genome. YACs clones of the CIC library carrying these minisatellites and minisatellite-like sequences were identified.Key words: Arabidopsis thaliana, minisatellites, polymorphism
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