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Journal of Experimental Botany, Vol 49, 27-32, Copyright © 1998 by Oxford University Press

ARTICLES

Hornet venom induces abundant expression of specific polypeptides in young barley leaves

M Metodiev
Institute of Plant Physiology, Bulgarian Academy of Science, G Bonche St., Bldg. 21, Sofia 1113, Bulgaria. E-mail: metodi@bgcict.acad.bg

Venom of Vespa crabro induced in barley an abundant expression of at least five polypeptides, having the same apparent molecular masses as the jasmonate-induced polypeptides. Treatment of venom by trypsin, as well as addition of indomethacin prevented the appearance of the polypeptides. Separation of the venom by gel-filtration chromatography showed that the fraction containing phospholipase activity was able to induce expression of the 23 kDa polypeptide as revealed by 2-D electrophoresis, but the induction was much stronger when this fraction was applied together with the fraction of the low molecular weight peptides. The treatment by venom did not promote senescence of the detached leaves as the jasmonate did and kept the photosynthesis, transpiration, protein content, and the intensity of labelled amino acid incorporation into proteins near the control values.Key words: Jasmonate-induced proteins, hornet venom, phospholipase, barley
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