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Journal of Experimental Botany, Vol 49, 33-39, Copyright © 1998 by Oxford University Press

ARTICLES

Triacylglycerol biosynthesis and gene expression in microspore-derived cell suspension cultures of oilseed rape

R Weselake, S Byers, J Davoren, A Laroche, D Hodges, M Pomeroy and T Furukawa-Stoffer
Department of Chemistry and Biochemistry, University of Lethbridge, Lethbridge, Alberta, Canada, T1J 4B1; Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada, T1J 4B1; Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada; Corresponding author at: weselake@uleth.ca

The effect of sucrose concentration on triacylglycerol biosynthesis and associated gene expression was examined in a microspore-derived cell suspension culture of oilseed rape (Brassica napus L. cv. Jet Neuf). The triacylglycerol content of the cells increased about 5-fold on a fresh weight basis when the sucrose concentration in the growth medium was raised from 2% to 22% (w/v). The specific activity of microsomal diacylglycerol acyltransferase (EC 2.3.1.20) and its activity per unit fresh weight increased about 2.5-fold and 6-fold, respectively, when sucrose concentration was increased from 2% to 14%. mRNA encoding the major oleosin also appeared to increase in abundance over the 2-14% sucrose concentration range when RNA fractions were analysed by the reverse transcription-polymerase chain reaction. The sucrose-mediated increases in diacylglycerol acyltransferase activity and oleosin mRNA indicated that the cell suspension could be a useful research tool for the identification of cDNAs encoding triacylglycerol biosynthetic enzymes and associated proteins.Key words: Triacylglycerol, Brassica, sucrose, acyltransferase, differential display.
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