Journal of Experimental Botany, Vol 49, 503-510, Copyright © 1998 by Oxford University Press
Z Fang, J Bouwkamp and T Solomos
The activities of chlorophyllase, contents of pigments including
chlorophyll a and b,
chlorophyllide a and b, and
phaeophorbide a during leaf senescence under low
oxygen (0.5% O2) and control (air) were investigated in a non-yellowing
mutant and wild-type leaves of snap beans (Phaseolus
vulgaris L.). Chlorophyllase from leaf tissues had maximum
activity when incubated at 40
ARTICLES
Chlorophyllase activities and chlorophyll degradation during leaf senescence in non-yellowing mutant and wild type of Phaseolus vulgaris L
Department of Horticulture, University of Maryland at College Park, MD 20742, USA; Corresponding author; Fax: +1 301 314 9308; E-mail: jb65@umail.umd.edu
C in a mixture
containing 50% acetone. In both mutant and wild type, chlorophyllase
activity was the highest in freshly harvested non-senescent leaves and
decreased sharply in the course of senescence, indicating that the loss of
chlorophylls in senescing leaves is not directly related to the activity of
chlorophyllase and that chlorophyllase activity is not altered in the
mutant. The wild type had higher ratios of chlorophyll
a to chlorophyll b than the
mutant and chlorophyll a : b ratios increased during
senescence in both types. In the senescent mutant leaves, accumulations of
chlorophyllide a and chlorophyllide
b were detected, but no phaeophorbide
a was found. Chlorophyllide b had
a greater accumulation than chlorophyllide a in the
early stage of senescence. Low oxygen treatment not only delayed
chlorophyll degradation but also enhanced the accumulations of
chlorophyllide a and b and
lowered the ratios of chlorophyll a to chlorophyll
b.Keywords: Chlorophyllase,
chlorophyll degradation, chlorophyllides, non-yellowing mutant,
Phaseolus vulgaris.
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