Journal of Experimental Botany, Vol 49, 511-520, Copyright © 1998 by Oxford University Press
M Williams, E Robertson, R Leech and J Harwood
Lipid synthesis was studied in primary leaves from 7-d-old wheat plants
which had been grown at either ambient CO2 concentration (350
ARTICLES
Lipid metabolism in leaves from young wheat Triticum aestivum cv. Hereward) plants grown at two carbon dioxide levels
School of Molecular and Medical Biosciences, University of Wales Cardiff, PO Box 911, Cardiff CF1 3US, UK; Department of Biology, The University of York, PO Box 373, York YO1 5YW, UK; Corresponding author; Fax: +44 1222 874 116
mol mol-1) or elevated CO2
(650 mol mol-1) by
incubating tissue samples with [1-14C]acetate.
Growth at different CO2 concentrations did not affect the total
incorporation of radiolabel into lipids but it did influence the relative
labelling of individual lipid classes, such as diacylglycerol. The leaf
basal segment was also studied separately and growth in an enriched CO2
atmosphere was associated with a dramatic increase (over 6-fold) in
diphosphatidylglycerol (cardiolipin) labelling, indicating an increased
rate of mitochondrial membrane biogenesis. Immunocytological observations
correlated with this metabolic result. Both leaf samples showed significant
decreases in pigment and surface wax labelling caused by growth at elevated
CO2.Growth at different CO2 concentrations also influenced fatty acid
labelling patterns, particularly those of the major labelled membrane
lipids of the primary leaf whereby there were changes in their ratios of
radiolabelled 16 carbon to 18 carbon fatty acids. Phosphatidylglycerol was
characterized, for instance, by increased palmitate labelling after wheat
was grown in elevated CO2 concentrations. In contrast, phosphatidylcholine
was marked by a dramatic decrease in palmitate labelling but a
corresponding increase in labelling of its 18 carbon unsaturated fatty
acids. The diacylglycerol fraction showed increased unsaturation of its C18
fatty acids. In addition, changes to the fatty acid moieties from the basal
segment lipids were also associated with changes in the amount of labelling
of the polyenoic fatty acids of monogalactosyldiacylglycerol. Possible
reasons for these changes in lipid labelling are discussed.The data show
that growth in elevated atmospheric CO2 concentrations causes significant
changes in the metabolism of leaf lipids as well as increasing
mitochondrial biogenesis.Keywords: Carbon dioxide
concentration, fatty acid composition, lipid synthesis, Triticum
aestivum, wheat.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Gregorini, K. J. Soder, and M. A. Sanderson Case Study: A Snapshot in Time of Fatty Acids Composition of Grass Herbage as Affected by Time of Day Professional Animal Scientist, December 1, 2008; 24(6): 675 - 680. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Wang, S. A. Heckathorn, D. Barua, P. Joshi, E. W. Hamilton, and J. J. LaCroix Effects of elevated CO2 on the tolerance of photosynthesis to acute heat stress in C3, C4, and CAM species Am. J. Botany, February 1, 2008; 95(2): 165 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. I. Hellgren and A. S. Sandelius Age-dependent variation in membrane lipid synthesis in leaves of garden pea (Pisum sativum L.) J. Exp. Bot., December 1, 2001; 52(365): 2275 - 2282. [Abstract] [Full Text] [PDF]
|
|