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Journal of Experimental Botany, Vol 49, 521-526, Copyright © 1998 by Oxford University Press

ARTICLES

Natural abundance of 15N in amino acids and polyamines from leguminous nodules: unique 15N enrichment in homospermidine

T Yoneyama, S Fujihara and K Yagi
Plant Nutrition Diagnosis Laboratory, National Agriculture Research Center (NARC), Kannondai 3-1-1, Tsukuba, Ibaraki 305, Japan; National Institute of Agro-Environmental Sciences, Kannondai 2-1-1, Tsukuba, Ibaraki 305, Japan; Corresponding author; Fax: +81 298 38 8837; E-mail: yoneyama@narc.affrc.go.jp

The natural 15N abundance (15N value) in acetylpropyl derivatives of amino acids and in ethyloxycarbonyl derivatives of polyamines was determined using a gas chromatography/combustion/mass spectrometer-(GC/C/MS). 15N value determined for 12 amino acids and five polyamines by GC/C/MS were identical to those obtained by a direct combustion method using an automatic nitrogen and carbon analysis (ANCA) mass spectrometer, the difference being less than 1.0% in most cases. The GC/C/MS method was used to analyse 15N values in the amino acids and polyamines from root nodules of pea and faba bean and from stem nodules of Sesbania rostrata. The analysis of 15N values revealed that homospermidine had high 15N values, as much as +40%, while the amino acids investigated had 15N values between -3 and +6%, putrescine between +2 and +8%, cadaverine between +1 and +7%, spermidine between -2 and +4%, and spermine between 0 and +6%. The mechanism of 15N enrichment in homospermidine is discussed.Keywords: Amino acid, legumes, natural abundance of 15N (15N), nodules, polyamines.
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