Journal of Experimental Botany, Vol 49, 535-546, Copyright © 1998 by Oxford University Press
H Hartel and B Grimm
Transgenic tobacco (Nicotiana tabacum), with a reduced
chlorophyll content of up to less than 10% of the wild-type level due to a
different expression of antisense RNA coding for glutamate 1-semialdehyde
aminotransferase, were used to study the relationship between chlorophyll
accumulation and changes in carotenoid composition in developing and mature
leaves grown either under low (30
ARTICLES
Consequences of chlorophyll deficiency for leaf carotenoid composition in tobacco synthesizing glutamate 1-semialdehyde aminotransferase antisense RNA: dependency on developmental age and growth light
Institut fur Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, D-06466 Gatersleben, Germany; Humboldt Universitat zu Berlin Institut fur Biologie/Pflanzenphysiologie, Philippstrasse 13, D-10115 Berlin, Germany; Corresponding author to be addressed at Gatersleben: Fax +49 39482 5139; E-mail: haertel@ipk-gatersleben.de
mol photons
m-2 s-1) or high light (300
mol photons m-2
s-1). Regardless of the extent to which chlorophyll
synthesis was reduced, under low light the ratios of total chlorophyll to
carotenoids remained constant. In contrast, under high light the content of
carotenoids was elevated relative to chlorophyll and increased further with
progressive inhibition in chlorophyll synthesis. The xanthophyll-cycle
pigment pool was most strongly increased (up to 18-fold) upon suppression
of chlorophyll synthesis. Concurrently to the higher pool sizes a higher
extent of violaxanthin was converted into antheraxanthin and zeaxanthin and
this was found to be correlated with a decrease in the quantum yield of
photosystem II photochemistry. While lutein increased (up to 3-fold) with
decreasing chlorophyll contents in high light transformants, neoxanthin
remained rather constant in all plants analysed. Based on the present
results, two different levels for the regulation of carotenoid synthesis
are proposed depending on (I) the chlorophyll synthesizing capacity, and
(ii) the photosynthetic light utilization efficiency. The first point
suggests a co-regulation between carotenoid and chlorophyll synthesis; the
second emphasizes the special role of carotenoids for protection against
light stress.Keywords: Light acclimation,
photoprotection, quantum yield, lutein, xanthophyll cycle.
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