Journal of Experimental Botany, Vol 49, 713-720, Copyright © 1998 by Oxford University Press
H Lopez-Delgado, J Dat, C Foyer and I Scott
Potato microplants propagated as nodal explants were subjected to heat
treatments in vitro similar to those employed in the
thermotherapy step of virus eradication procedures. Low concentrations
(10-6-10-5 M) of
acetylsalicylic acid (ASA) in the culture medium improved (by 3.7-fold)
tolerance of a 5-week high-temperature (35
ARTICLES
Induction of thermotolerance in potato microplants by acetylsalicylic acid and H2O2
Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion SY23 3DA, UK; Environmental Biology Department, Institute of Grassland and Environmental Research, Aberystwyth, Ceredigion SY23 3EB, UK; Present address: Programa de Papa, Instituto Nacional de Investigaciones Forestales y Agropecuarias (INIFAP), Metepec, Mexico City 52142, AP 1-2, Mexico; Corresponding author; e-mail: ias@aber.ac.uk
C)
treatment. Furthermore, tissues subcultured on to ASA-free medium following
several weeks of growth on ASA were more thermotolerant (by 3.8-fold) of a
7 week 35C treatment, and (by 38-fold) of a
15 h 42°C heat-shock. Stems of microplants grown on ASA contained
significantly less catalase activity and higher levels of H2O2 than
controls. Explanting and heat treatment, however, reduced catalase activity
to similar levels in ASA-treated and control microplant tissues. To
investigate whether H2O2 could be involved in signal transduction during
the induction of thermotolerance, nodal explants were incubated for 1 h in
H2O2 (0.1-50 mM), and then cultured under standard conditions. The
microplants that grew from the H2O2-treated explants showed
concentration-dependent decreases in stem height, but were significantly
more thermotolerant than controls, more than 1 month after the H2O2
treatment. Thus, thermotolerance induced in these conditions was extremely
stable. It is concluded that both salicylate and H2O2 treatments can
induced thermotolerance in this system.Keywords:
Acetylsalicylic acid, heat-shock, hydrogen peroxide, potato,
microplant, thermotolerance.
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