Journal of Experimental Botany, Vol 49, 731-737, Copyright © 1998 by Oxford University Press
E Cecchini, N Al-Kaff, A Bannister, M Giannakou, D McCallum, A Maule, J Milner and S Covey
Pathogenic interactions between genetic variants of cauliflower mosaic
virus (CaMV) and Arabidopsis thaliana were
characterized to identify combinations potentially useful in molecular
genetic analysis. Infections of a glabrous mutant
(gl1) of Arabidopsis ecotype
Columbia (Col-0 gl1) by 30 CaMV isolates were assessed
by recording symptom character. Thirteen isolates failed to cause symptoms;
the remainder induced symptoms that varied between mild and very severe.
Some CaMV isolates produced symptoms in Arabidopsis
that differed significantly in severity or character from those produced in
a standard host Brassica rapa (turnip). A greater
variety of symptom types was observed in a single
Arabidopsis ecotype infected with a range of CaMV
isolates than was found in a range of Arabidopsis
ecotypes infected with a single, typical CaMV isolate (Cabb B-JI). One
isolate, Bari-1, that was asymptomatic but accumulated virus in
Arabidopsis ecotype Col-0 gl1,
caused mild symptoms in ecotype Ler gl1. A hybrid
virus constructed from CaMV isolates Cabb B-JI and Bari-1 produced symptoms
in Arabidopsis variants that were more severe than in
either parental isolate. From a screen of EMS-mutagenized
Arabidopsis, one mutant (Col-0
dv1) with a pale-green, dark-vein phenotype which had
an altered symptom response to CaMV, was isolated. From this, a
phenotypically near-normal revertant (Col-0 dv1R)
spontaneously arose, but which showed altered responses to CaMV. Infection
of Col-0 dv1R by CaMV isolate Bari-1 elicited symptoms
unlike the parent Arabidopsis ecotype (Col-0
gl1). Also, Col-0 dv1 and Col-0
dv1R expressed an uncharacteristic necrotic reaction
to CaMV.Keywords: Caulimovirus, Cruciferae, plant
mutants, symptom expression.
ARTICLES
Pathogenic interactions between variants of cauliflower mosaic virus and Arabidopsis thaliana
Plant Molecular Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, Glasgow University, Glasgow G12 8QQ, UK; Department of Virus Research, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK; Corresponding author; e-mail: covey@bbsrc.ac.uk
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. C. Roden and R. A. Ingle Lights, Rhythms, Infection: The Role of Light and the Circadian Clock in Determining the Outcome of Plant-Pathogen Interactions PLANT CELL, September 1, 2009; 21(9): 2546 - 2552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Love, J. Laird, J. Holt, A. J. Hamilton, A. Sadanandom, and J. J. Milner Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing J. Gen. Virol., December 1, 2007; 88(12): 3439 - 3444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Handford and J. P. Carr A defect in carbohydrate metabolism ameliorates symptom severity in virus-infected Arabidopsis thaliana J. Gen. Virol., January 1, 2007; 88(1): 337 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Love, B. W. Yun, V. Laval, G. J. Loake, and J. J. Milner Cauliflower mosaic virus, a Compatible Pathogen of Arabidopsis, Engages Three Distinct Defense-Signaling Pathways and Activates Rapid Systemic Generation of Reactive Oxygen Species Plant Physiology, October 1, 2005; 139(2): 935 - 948. [Abstract] [Full Text] [PDF]
|
|