Journal of Experimental Botany, Vol 49, 789-795, Copyright © 1998 by Oxford University Press
J Gallagher and C Pollock
A novel cDNA clone, functionally expressed in E. coli,
was isolated from a L. temulentum L. cDNA library. The
expressed protein hydrolysed sucrose with an apparent
Km of approximately 18 mM, and produced equi-molar
concentrations of glucose and fructose. Optimum activity was observed at pH
7-7.5; there was little or no activity at pH 5.5. The expressed protein did
not hydrolyse raffinose, stachyose or maltose. The activity of the
expressed protein was inhibited by fructose (50% at 15 mM) and TRIS (50% at
2.5 mM), but was not affected by MgCl2, CaCl2 or MnCl2. These findings
suggest that this cDNA clone encodes for an alkaline/neutral invertase.
Sequence analysis revealed little homology with published sequences for
acid invertase, however the invertase motif (NDPN) identified in other
invertases was present. Expression studies show that the gene encoding for
this enzyme is not regulated by sucrose accumulation in leaf
tissue.Keywords: cDNA clone, Lolium
temulentum L, sucrose hydrolysis, invertase activity.
ARTICLES
Isolation and characterization of a cDNA clone from Lolium temulentum L. encoding for a sucrose hydrolytic enzyme which shows alkaline/neutral invertase activity
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion SY23 3EB, UK; Corresponding author; e-mail: joe.gallagher@bbsrc.ac.uk
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Skot, J. Humphreys, M. O. Humphreys, D. Thorogood, J. Gallagher, R. Sanderson, I. P. Armstead, and I. D. Thomas Association of Candidate Genes With Flowering Time and Water-Soluble Carbohydrate Content in Lolium perenne (L.) Genetics, September 1, 2007; 177(1): 535 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Gallagher, A. J. Cairns, and C. J. Pollock Cloning and characterization of a putative fructosyltransferase and two putative invertase genes from the temperate grass Lolium temulentum L. J. Exp. Bot., March 1, 2004; 55(397): 557 - 569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Gout, A.-M. Boisson, S. Aubert, R. Douce, and R. Bligny Origin of the Cytoplasmic pH Changes during Anaerobic Stress in Higher Plant Cells. Carbon-13 and Phosphorous-31 Nuclear Magnetic Resonance Studies Plant Physiology, February 1, 2001; 125(2): 912 - 925. [Abstract] [Full Text]
|
|
|
|
|
|
A. Sturm Invertases. Primary Structures, Functions, and Roles in Plant Development and Sucrose Partitioning Plant Physiology, September 1, 1999; 121(1): 1 - 8. [Full Text]
|
|
|
|
|
|
A. J. Gordon, F. R. Minchin, C. L. James, and O. Komina Sucrose Synthase in Legume Nodules Is Essential for Nitrogen Fixation Plant Physiology, July 1, 1999; 120(3): 867 - 878. [Abstract] [Full Text]
|
|