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Journal of Experimental Botany, Vol 49, 1259-1262, Copyright © 1998 by Oxford University Press

ARTICLES

Short communication. Localization of {alpha}-tocopherol synthesis in chromoplast envelope membranes of Capsicum annuum L. fruits

Y Arango and K Heise
Albrecht-von-Haller-Institut fur Pflanzenwissenschaften der Universitat Gottingen, Abteilung Biochemie der Pflanze, Untere Karspule 2, D-37073 Gottingen, Germany; Corresponding author; e-mail: yarango@gwdg.de

Chromoplast membranes from Capsicum fruits were fractionated by isopycnic continuous sucrose gradients resulting in the separation of outer (d = 1.08 g cm-3) and inner (d = 1.11 g cm-3) envelopes. Both membranes were characterized by marker enzymes and with antibodies against the phosphate translocator. This report is concerned with the location of -tocopherol synthesis in these membranes.Keywords: Capsicum fruits, chromoplasts, -tocopherol synthesis, compartmentation, envelope membranes.
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