Journal of Experimental Botany, Vol 49, 1361-1370, Copyright © 1998 by Oxford University Press
D Bussis and D Heineke
Potato plants (Solanum tuberosum cv.
Désirée) were grown hydroponically and subjected to
water deficit induced by addition of 10% (w/v) PEG 6000. The potato plants
were able to grow under water deficit by accumulating organic solutes
(osmoregulation). Osmoregulation occurred in two phases. During the initial
2d hexoses were accumulated, and after 7 d of PEG treatment osmotic
adjustment was mostly due to the accumulation of amino acids, especially
proline, which accumulated up to 150 times the control content. Sucrose
contents remained unchanged in leaves of PEG-treated plants compared with
controls, whereas the starch content decreased during PEG treatment.In
control leaves, the hexoses and malate were compartmented in the vacuole
and sucrose was found in the cytosol and vacuole. Amino acids were
distributed between the cytosol and stroma, but only minor amounts of amino
acids could be detected in the vacuole. Under water deficit the subcellular
distribution of hexoses, malate and sucrose remained unchanged. Most amino
acids showed a slight to moderate higher concentration in the vacuole under
water deficit. Proline, the metabolite contributing mainly to
osmoregulation, was concentrated mostly in the chloroplast and the cytosol.
This underlines the important role of proline as the osmolyte under water
deficit.Keywords: Amino acids, osmoregulation,
proline, water stress, Solanum tuberosum L.
ARTICLES
Acclimation of potato plants to polyethylene glycol-induced water deficit II. Contents and subcellular distribution of organic solutes
Albrecht-von-Haller Institut fur Pflanzenwissenschaften, Abteilung Biochemie der Pflanze, Untere Karspule 2, D-37073 Gottingen, Germany; Present address: Molecular Plant Physiology Group, Research School of Biological Sciences, Australian National University, PO Box 475, Canberra, ACT 2601, Australia; Corresponding author
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Grallath, T. Weimar, A. Meyer, C. Gumy, M. Suter-Grotemeyer, J.-M. Neuhaus, and D. Rentsch The AtProT Family. Compatible Solute Transporters with Similar Substrate Specificity But Differential Expression Patterns Plant Physiology, January 1, 2005; 137(1): 117 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Jiang and B. Huang Osmotic Adjustment and Root Growth Associated with Drought Preconditioning-Enhanced Heat Tolerance in Kentucky Bluegrass Crop Sci., July 1, 2001; 41(4): 1168 - 1173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hellmann, D. Funck, D. Rentsch, and W. B. Frommer Hypersensitivity of an Arabidopsis Sugar Signaling Mutant toward Exogenous Proline Application Plant Physiology, June 1, 2000; 123(2): 779 - 789. [Abstract] [Full Text]
|
|
|
|
|
|
H. Hellmann, D. Funck, D. Rentsch, and W. B. Frommer Hypersensitivity of an Arabidopsis Sugar Signaling Mutant toward Exogenous Proline Application Plant Physiology, February 1, 2000; 122(2): 357 - 368. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Schwacke, S. Grallath, K. E. Breitkreuz, E. Stransky, H. Stransky, W. B. Frommer, and D. Rentsch LeProT1, a Transporter for Proline, Glycine Betaine, and {gamma}-Amino Butyric Acid in Tomato Pollen PLANT CELL, March 1, 1999; 11(3): 377 - 392. [Abstract] [Full Text] [PDF]
|
|