Journal of Experimental Botany, Vol 49, 1637-1646, Copyright © 1998 by Oxford University Press
O Ueno
Cellular localization of photosynthetic enzymes was investigated by
immunogold electron microscopy for leaves of nine C4 grasses (three
NADP-malic enzyme (NADP-ME)subtype species, three NAD-malic enzyme (NAD-ME)
subtype species, and three phosphoenolpyruvate carboxykinase (PCK) subtype
species), two C4 sedges (NADP-ME subtype species) and two C4 dicots (an
NADP-ME and an NADP/NAD-ME subtype species). In leaves of all species,
immunogold labelling was present for phosphoenolpyruvate carboxylase in the
cytosol of the mesophyll cells (MC) and for ribulose-1,5-bisphosphate
carboxylase/oxygenase in the chloroplasts of the bundle sheath cells (BSC).
However, considerable specific variation was found in the intercellular
patterns of labelling for pyruvate orthophosphate dikinase (PPDK). In the
NADP-ME grasses, two NAD-ME grasses, and the dicots, significant labelling
for PPDK was present in the both the BSC and the MC chloroplasts. In the
other NAD-ME grass, the PCK grasses, and the sedges, labelling for PPDK was
present almost exclusively in the chloroplasts of the MC. These patterns
were observed in the leaves of both young seedlings and mature plants.
These results indicate that the accumulation of PPDK in leaves of C4 plants
is not necessarily restricted to the MC, although the chloroplasts of the
MC accumulate more than those of the BSC.Key words: C4 plants,
immunolocalization, phosphoenolpyruvate carboxylase, pyruvate
orthophosphate dikinase, ribulose-1,5-bisphosphate carboxylase/oxygenase.
ARTICLES
Immunogold localization of photosynthetic enzymes in leaves of various C4 plants, with particular reference to pyruvate orthophosphate dikinase
Department of Plant Physiology, National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305, Japan
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