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Journal of Experimental Botany, Vol 49, 1773-1774, Copyright © 1998 by Oxford University Press

ARTICLES

Gene note. Isolation of a cDNA clone corresponding to a protein kinase differentially expressed in the resurrection plant Craterostigma plantagineum

P Heino, M Nylander, T Palva and D Bartels
Max-Planck-Insitut fur Zuchtungsforschung, Carl von Linee Weg 10, D-50829 Koln, Germany; Department of Biosciences, Division of Genetics, University of Helsinki, Box 56, FIN-00014, Helsinki, Finland; Department of Plant Biology, Swedish University of Agricultural Sciences, Box 7010, S-75007 Uppsala, Sweden

A cDNA clone corresponding to a putative serine/threonine kinase (cppk1) was isolated from the resurrection plant Craterostigma plantagineum. The predicted amino acid sequences exhibited high similarity to previously described protein kinases in higher plants. Northern blot analysis showed that the corresponding gene was expressed both in leaves and in callus tissue of Craterostigma. When the tissues were desiccated the amount of the transcript was heavily reduced in leaves whereas no effect was found in callus. ABA treatment had no effect on the accumulation of the transcript.Key words: Abscisic acid, desiccation tolerance, protein kinase, resurrection plant.
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