Journal of Experimental Botany, Vol 49, 1797-1804, Copyright © 1998 by Oxford University Press
A Bettany, S Dalton, E Timms and P Morris
The expression of a
ARTICLES
Stability of transgene expression during vegetative propagation of protoplast-derived tall fescue (Festuca arundinacea Schreb.) plants
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion, Wales SY23 3EB, UK; Corresponding author e-mail: andrew.bettany@bbsrc.ac.uk
-glucuronidase (GUS)
transgene, under the control of the rice actin1
promoter and first intron, introduced via PEG-mediated transformation of
protoplasts, has been followed through a number of generations of
vegetative propagation (tillering) in the forage grass Festuca
arundinacea. Gene expression was found to be particularly
unstable during early generations of tillering, but more stable in the
fourth or fifth tiller generation. Loss of transgene expression was not due
to deletion of the transgene nor was it due to gross rearrangements in the
transgene as demonstrated by Southern blot analysis of expressing and
non-expressing tillers. Loss of transgene expression due to
hypermethylation of the transgene promoter has been shown previously, but
in this case it was not possible to detect differences in the methylation
patterns of the coding and promoter regions of expressing and
non-expressing tillers. The implications for screening of transgenic plants
intended to be vegetatively propagated are discussed.Key words:
Festuca arundinacea, forage grass, transgene expression,
vegetative propagation.
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