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Journal of Experimental Botany, Vol 49, 1893-1894, Copyright © 1998 by Oxford University Press


ARTICLES

Gene note. Cloning of a wilt-responsive cDNA from an Arabidopsis thaliana suspension culture cDNA library that encodes a putative 9-cis-epoxy-carotenoid dioxygenase

S Neill, E Burnett, R Desikan and J Hancock
Department of Biological and Biomedical Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK; Corresponding author e-mail: Steen.Neill@uwe.ac.uk

This report describes the cloning of a cDNA from an Arabidopsis thaliana suspension culture cDNA library that encodes a potential 9-cis-epoxy-carotenoid dioxygenase, a key enzyme involved in the biosynthesis of abscisic acid. The predicted protein sequence of this cDNA, termed AtNCED1, shares conserved regions with those of published epoxy-carotenoid dioxygenase enzymes from maize and tomato. At NECD1 mRNA was present in turbid shoot tissues and rapid dehydration resulted in accumulation of AtNCED1 mRNA.Key words: ABA biosynthesis, Arabidopsis thaliana, 9-cis-epoxy-carotenoid dioxygenase.
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