Journal of Experimental Botany, Vol 49, 1935-1944, Copyright © 1998 by Oxford University Press
M Sturaro, C Linnestad, A Kleinhofs, O Olsen and D Doan
A partial cDNA clone Hvex1 from Hordeum
vulgare L. encoding a putative hydroxyproline-rich protein of
the extensin family was isolated in an experiment designed to identify
transcripts differentially expressed in the coenocytic endosperm and the
surrounding sporophytic tissues during the early stages of grain
development. The amino acid sequence derived from the
Hvex1 cDNA has a high proportion of Pro, Lys and Thr
residues, and a pl of approximately 11. However, the deduced
Hvex1 polypeptide has an unusual structure unlike
those of known monocot extensins and consists of four domains with repeats
of the sequences KPP, PKPAPPTY(K/S)P, SPPAYKPAPKV, and (H/Y)KPPTPTPPA,
respectively, ad a fifth domain with a single SPPPP motif. In
situ hybridization and Northern analyses reveal that
Hvex1 transcripts are expressed in the nucellus, the
nucellar epidermis and the nucellar projection of developing barley grains.
In addition to nucellar tissues, the Hvex1 transcript
is also detected in the vascular tissue of the pericarp, scutellum of the
developing embryo, stigma, and root tips. The Hvex1
transcript is encoded by a single gene located near the centromere of
barley chromosome 2.Key words: Extensin,
hydroxyproline-rich glycoprotein, nucellus, Hordeum
vulgare.
ARTICLES
Characterization of a cDNA encoding a putative extensin from developing barley grains (Hordeum vulgare L.)
Plant Molecular Biology Laboratory, Department of Biotechnological Sciences, Agricultural University of Norway, Aas 1432, Norway; Present address: Istituto Biosintesi Vegetali, CNR, Milano 21033, Italy; Present address; Department of Crop and Soil Sciences, Washington State University, Pullman WA 99164-6420, USA; Corresponding author at: Institute of Molecular Agrobiology, 1 Research Link, National University of Singapore, Singapore 117604. E-mail: doan@ima.org.sg
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