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Journal of Experimental Botany, Vol 50, 139-140, Copyright © 1999 by Oxford University Press

ARTICLES

Gene note. A vacuolar H+-pyrophosphatase in Acetabularia acetabulum: molecular cloning and comparison with higher plants and a bacterium

M Ikeda, M Rahman, C Moritani, K Umami, Y Tanimura, R Akagi, Y Tanaka, M Maeshima and Y Watanabe
Faculty of Health and Welfare Science, Okayam Prefectural University, Kuboki 111, Soja 719-11, Japan; Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka-1, Okayama 700, Japan; Chemical Tolerance, National Institute of Agrobiological Resources, Tsukuba Ibaraki 305, Japan; Faculty of Agricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-01, Japan; Corresponding author; Fax: +81 866 94 2152; E-mail: mikieda@fhw.oka-pu.ac.jp

An almost complete cDNA clone [pPPI (AcVP gene), 3104 bp in length/open reading frame from 795 to 2960 bp/721 amino acids with a molecular weight of 74 406] encoding Acetabularia H+-translocating inorganic pyrophosphatase was isolated from total RNA using reverse-transcription and PCR techniques. Alignments of the primary structure to that of similar enzymes in higher plants and Rhodospirillum rubrum revealed that overall similarities were around 50%, the C-terminal half was well conserved but the N-terminal half showed divergencies except in a postulated substrate-binding domain. By Northern analysis, about 3.2 kb RNA mainly hybridized with 5-untranslated region specific RNA probe of the AcVP gene.Keywords: inorganic pyrophosphatase, vacuole, H+-translocation, molecular cloning, Acetabularia acetabulum
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