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Journal of Experimental Botany, Vol 50, 71-78, Copyright © 1999 by Oxford University Press

ARTICLES

Effects of overproduction of tobacco MnSOD in maize chloroplasts on foliar tolerance to cold and oxidative stress

F Van Breusegem, L Slooten, J Stassart, J Botterman, T Moens, M Van Montagu and D Inze
Laboratorium voor Genetica, Department of Genetics, Flanders Interuniviersity Institute for Biotechnology (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium; Laboratorium voor Biofysica, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium; Plant Genetic Systems N.V., Plateaustraat 22, B-9000 Gent, Belgium; Laboratoire Associe de l'Institut National de la Recherche Agronomique (France), Universiteit Gent, B-9000 Gent, Belgium; Corresponding author; Fax: +32 9 264 5349; E-mail: mamon@gengenp.rub.ac.be

Transgenic maize plants have been generated by particle gun bombardment that overproduce a Nicotiana plumbaginifolia L. manganese superoxide dismutase (MnSOD). To target this mitochondrial enzyme into chloroplasts, the mature MnSOD-coding sequence was fused to a chloroplast transit peptide from a pea ribulose-1,5-bisphosphate carboxylase gene, whereas expression of the chimeric gene was driven by the CaMB 35S promoter. Transgenic MnSOD activity contributed to 20% of the total SOD activity. The presence of transgenic MnSOD had clear effects on foliar tolerance to chilling and oxidative stress. The results suggest that overproduction of MnSOD in the chloroplasts increases the antioxidant capacity of the leaves.Keywords: Chilling stress, oxidative stress, superoxide dismutase, transgenic plants, Zea mays L.
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