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Journal of Experimental Botany, Vol 50, 311-317, Copyright © 1999 by Oxford University Press

ARTICLES

Reduced aleurone {alpha}-amylase production in aged wheat seeds is accompanied by lower levels of high-pI -amylase transcripts and reduced response to gibberellic acid

I Bernal-Lugo, M Rodriguez, M Gavilanes-Ruiz and A Hamabata
Departamento de Bioquimica, Facultad de quimica, UNAM, Mexico D.F. 04510; Departamento de Bioquimica CINVESTAV-IPN, 07000, Mexico D.F.; Corresponding author e-mail: irmofel@servicor.unam.mx

With the aim of determining the level at which ageing exerts its effect on the expression of -amylase, GA3 regulation of -amylase production was studied in isolated aleurone layers from aged wheat seeds. GA3-induced -amylase activity was lower in the tissue from aged seeds than in controls. However, the proportion of 35S-methionine incorporated into -amylase was higher in the aged than in control tissue. The pattern of -amylase isoforms was resolved by isoelectric focusing and showed that two isogroups were present with the activity of the high-pI isogroup being higher in the control than in the aged lot. These apparently contradictory results may be explained in terms of differences in isozyme expression. Studies on the expression of -amylase genes indicated a reduction in the level of high-pI mRNA in aged tissue. Dose-response curves showed lower GA3-responsiveness of aleurone layers from aged seeds as compared to the controls. From these results, it is proposed that the diminished capacity of -amylase production in aleurone from aged seeds is apparently due to a decrease in the expression of the high-pI -amylase genes, and this reduction is associated with a decrease in the response to GA3.Key words: Seed ageing, wheat aleurone, gibberellic acid, -amylase isozymes, gene expression.
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