Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Hirsinger, C.
Right arrow Articles by Jamet, E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Hirsinger, C.
Right arrow Articles by Jamet, E.
Agricola
Right arrow Articles by Hirsinger, C.
Right arrow Articles by Jamet, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol 50, 343-355, Copyright © 1999 by Oxford University Press

ARTICLES

The tobacco extensin gene Ext 1.4 is expressed in cells submitted to mechanical restraints and in cells proliferating under hormone control

C Hirsinger, , , J Marbach, A Durr, J Fleck and E Jamet
Institut de Biologie Moléculaire des Plantes, UPR CNRS A0406, 12 rue du Général Zimmer, 67000 Strasbourg, France; Corresponding author e-mail: elisabeth.jamet@bmp-ulp.u-strasb.fr

The promoter region of a Nicotiana tabacum extensin gene (Ext 1.4) was studied in tobacco transgenic plants carrying Ext 1.4/GUS (-glucuronidase) chimeric genes. The pattern of expression could be defined and cis-regulatory elements were localized in small regions of the promoter. In healthy plants, expression was essentially found in cells under mechanical stress, that is at the emergence of lateral roots, at the junction between stem and petiole and at the fusion of carpels. In roots of germinating plantlets, expression was found in the piliferous zone. In flowers, expression was found on the one hand in the placenta, in the locular tissue of ovaries and in the zone of carpel fusion, and on the other hand in the connective tissue of anthers, in mature and in germinating pollen. A developmental regulation during seed germination, where the gene fusion is transiently expressed in the endosperm and in the root tip before its expression becomes similar to that found in mature plants has also been shown. The expression of the Ext 1.4/GUS chimeric gene was also induced during cell proliferation under hormone control, for example in response to Agrobacterium tumefaciens infection and in calli. However, when organogenesis occurred under hormone control, expression was never found in root or shoot primordia. Cis-regulatory elements important for expression of the Ext 1.4 GUS gene fusion in germinating seeds, in mature plants or in proliferating cells have been localized in the proximal promoter region whereas enhancer elements have been located further upstream.Key words: Cell proliferation, extensin, Nicotiana tabacum, promoter, protoplast.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.