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Journal of Experimental Botany, Vol 50, 785-791, Copyright © 1999 by Oxford University Press

ARTICLES

Overexpression of sucrose phosphate synthase increases sucrose unloading in transformed tomato fruit

B Nguyen-Quoc, H N'Tchobo, C Foyer and S Yelle
Centre de Recherche en Horticulture, Pavillon de l'Envirotron, FSAA, Universityé Laval, Québec G1K 7P4, Canada; Department of Biochemistry and Physiology, Institute of Arable Crops Research, Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK; Corresponding author; Fax: +44 1582 763010; E-mail: christine.foyer@bbsrc.ac.uk

Sucrose unloading and sink activity were examined in tomato plants (Lycopersicon esculentum) overexpression sucrose phosphate synthase (SPS; EC 2.3.1.14). Like the leaves, the fruit of the transformed tomato plants had elevated (2.4-fold) SPS activity. SPS over-expression in tomato fruit did not significantly change acid invertase, and only slightly reduced ADPglc ppase activity, but enhanced sucrose synthase activity by 27%. More importantly, the amount of sucrose unloaded into the fruit was considerably increased. Using [3H]- (fructosyl)-sucrose in in vitro unloading experiments with harvested 20-d-old fruit, 70% more sucrose was unloaded into the transformed fruits compared to the untransformed controls. Furthermore, the turnover of the sucrose unloaded into the fruit of transformed plants was 60% higher than that observed in the untransformed controls. Taken together, these results demonstrate that SPS overexpression increases the sink strength of transformed tomato fruit.Keywords: Sucrose phosphate synthase (SPS), starch, sucrose unloading, tomato fruit, carbon metabolism.
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