Journal of Experimental Botany, Vol 50, 793-798, Copyright © 1999 by Oxford University Press
A Griffiths, C Barry, A Alpuche-Solis and D Grierson
The role of ethylene in regulating the expression of three lipoxygenase
(LOX) genes TomloxA, TomloxB and
TomloxC during fruit ripening has been investigated.
The study used a combination of low-ethylene fruit containing an ACC
oxidase (ACO1) sense-suppressing transgene and tomato
fruit ripening mutants deficient in ethylene perception in the case of
Never-ripe (Nr) or ripening in
the case of the ripening-inhibitor
(rin). The results indicate that the three genes are
differentially regulated during fruit ripening and that a combination of
ethylene and developmental factors influence their expression. The
expression of TomloxA declines during ripening and
this is delayed in the ACO1 transgenic low-ethylene
and Nr fruit indicating that this phenomenon is
ethylene regulated. However, transcript abundance also declines during
rin fruit development indicating that developmental
factors also influence the expression of TomloxA.
TomloxB expression is increased by ethylene as reduced
accumulation is evident in transgenic and mutant fruit. The same ethylene
up-regulated and ripening-related expression is shown by
TomloxC, however, expression increases in
rin fruit at the onset of colour change, indicating
that there may also be a developmental component regulating
TomloxC expression. These results indicate that
individual LOX isoforms are differentially regulated and may have distinct
functions during tomato fruit ripening.Keywords:
Lipoxygenase, ethylene, fruit ripening, gene expression, tomato
mutants.
ARTICLES
Ethylene and developmental signals regulate expression of lipoxygenase genes during tomato fruit ripening
Plant Science Division, School of Biological Sciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK; Present address: Unilever Research Colworth Laboratory, Colworth House, Sharnbrook, Bedford MK44 1LQ, UK; Corresponding author; Fax: +44 115 951 6334; E-mail: Donald. Grierson@nottingham.ac.uk
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