Journal of Experimental Botany, Vol 50, 1299-1306, Copyright © 1999 by Oxford University Press
S Quarrie, V Lazic-Jancic, D Kovacevic, A Steed and S Pekic
The usual method to locate and compare loci regulating quantitative traits
(QTLs) requires a segregating population of plants with each one genotyped
with molecular markers. However, plants from such segregating populations
can also be grouped according to phenotypic expression of a trait and
tested for differences in allele frequency between the population bulks:
bulk segregant analysis (BSA). The same probes used for making a genetic
map (e.g. isozyme, RFLP, RAPD, etc) can be used for BSA. A molecular marker
showing polymorphism between the parents of the population and which is
closely-linked to a major QTL regulating a particular trait will mainly
co-segregate with that QTL, i.e. segregate according to the phenotype if
the QTL has a large effect. Thus, if plants are grouped according to
expression of the trait and extreme groups tested with that polymorphic
marker, the frequency of the two marker alleles present within each of the
two bulks should deviate significantly from the ratio of 1 : 1 expected for
most populations. As chromosomal locations of many molecular markers have
now been determined in many species, the map location of closely-linked
QTLs can therefore be deduced without having to genotype every individual
in segregating populations. This has been used successfully with composite
populations of maize to locate QTLs associated with yield under severe
drought. An inbred line derived from one of the populations selected for
higher drought yield has been crossed with a drought-susceptible inbred
line to produce a mapping population for QTL analysis of physiological and
developmental traits likely to regulate yield under drought. Future work to
identify traits having QTLs with flanking markers showing significant
allele frequency differences in the GSA studies will indicate those traits
likely to be important in determining yield under drought.Key
words: Bulk segregant analysis (BSA), drought resistance,
genetic maps, maize, molecular markers, Zea mays (L.).
ARTICLES
Bulk segregant analysis with molecular markers and its use for improving drought resistance in maize
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK; Maize Research Institute, Slobodana Bajica 1, Zemun Polje, 11080 Belgrade-Zemun, Yugoslavia; Faculty of Agriculture, University of Belgrade, PO Box 127, Nemanjina 6, 1181 Belgrade-Zemun, Yugoslavia; Corresponding author e-mail: quarrie@bbsrc.ac.uk
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