Journal of Experimental Botany, Vol 50, 1307-1313, Copyright © 1999 by Oxford University Press
H Nonogaki and Y Morohashi
The development of endo-
ARTICLES
Temporal and spatial pattern of the development of endo-{beta}-mannanase activity in germinating and germinated lettuce seeds
Department of Regulatory Biology, Faculty of Science, Saitama University, Urawa, Saitama 338-8570, Japan; Present address: Department of Vegetable Crops, University of California, Davis, CA 95616-8631, USA; Corresponding author e-mail: moro@sacs.sv.saitama-u.ac.jp
-mannanase activity
and the changes in the enzyme content were followed during and after
germination of lettuce seeds. Endo--mannanase activity was not detected before
germination. It began to develop immediately after radicle protrusion. The
development of the enzyme activity occurred specifically in the endosperm
tissue and activity staining of native-PAGE gels revealed that three
isoforms of the enzyme are present. SDS-denatured polypeptides of these
isoforms were all recognized by anti-tomato-endo-
-mannanase
antibodies. Molecular masses of these polypeptides seemed to be very close
to each other and were estimated to be about 39 kDa. The pattern of changes
in the activity of endo--mannanase in the endosperm during
seedling growth was parallel to that of changes in the content of the
enzyme protein, indicating that the increase in enzyme activity is due to
the accumulation of the enzyme protein. Tissue prints showed that the
activity initially developed in the endosperm region near the embryonic
axis and then spread over the endosperm tissue. These results indicate that
endo--mannanase production in lettuce endosperm is carried out in
a spatially and temporally regulated manner.Key words:
Endo--mannanase, Lactuca sativa, lettuce,
seed germination, tissue printing
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