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Journal of Experimental Botany, Vol 50, 1421-1422, Copyright © 1999 by Oxford University Press

ARTICLES

Gene note. Complete nucleotide sequence of the T-DNA region of the plant tumour-inducing Agrobacterium tumefaciens Ti plasmid pTiC58

J Gielen, N Terryn, R Villarroel and M Van Montagu
Laboratorium voor Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium; Corresponding author e-mail: mamon@gengenp.rug.ac.be

The complete nucleotide sequence has been determined of the T-DNA region from the plant tumour-inducing Agrobacterium tumefaciens nopaline Ti plasmid pTiC58. The T-DNA itself consists of 24 782 bp flanked by two direct 25 bp repeats, the border sequences. In addition, 3622 bp located at the left and 1070 bp at the right of the T-DNA borders were sequenced. Twenty-two open reading frames that code for proteins larger than 125 amino acids have been identified.Key words: Agrobacterium tumefaciens, sequence, T-DNA.
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