Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Green, L.
Right arrow Articles by Emerich, D.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Green, L.
Right arrow Articles by Emerich, D.
Agricola
Right arrow Articles by Green, L.
Right arrow Articles by Emerich, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol 50, 1577-1585, Copyright © 1999 by Oxford University Press

ARTICLES

Light microscopy of early stages in the symbiosis of soybean with a delayed-nodulation mutant of Bradyrhizobium japonicum

L Green and D Emerich
Biochemistry Department, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA; Corresponding author; Fax: +1 314 882 5635; E-mail: greenl@missouri.edu

An -ketoglutarate dehydrogenase mutant (LSG184) of Bradyrhizobium japonicum USDA110 has a delayed nodulation phenotype when inoculated onto soybean (Glycine max L.). To pinpoint the defective stage of symbiotic development, light microscopic techniques were used to monitor early responses of soybean to inoculation with the mutant as compared to the wildtype strain. Methylene blue was used to visualize curled root hairs and a convenient haematoxylin staining method was developed that could detect nodule primordia as early as 2 d after inoculation. The results demonstrate that early symbiotic events occur with normal timing after inoculation with SLG184 and that its developmental delay is first evident during the progression of nodule primordia into emergent nodules. The timing of this delay suggests that LSG184 is not deficient in Nod factor production, at least during the early stages of symbiosis, but rather may have a defect in infection thread initiation or elongation. The results further imply that the rate of development of advanced soybean nodule primordia is, in part, dependent on the metabolic capabilities of the invading bacterium.Keywords: Soybean, nodulation, light microscopy, haematoxylin, symbiosis.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
R. Shah and D. W. Emerich
Isocitrate Dehydrogenase of Bradyrhizobium japonicum Is Not Required for Symbiotic Nitrogen Fixation with Soybean
J. Bacteriol., November 1, 2006; 188(21): 7600 - 7608.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
L. S. Green, Y. Li, D. W. Emerich, F. J. Bergersen, and D. A. Day
Catabolism of alpha -Ketoglutarate by a sucA Mutant of Bradyrhizobium japonicum: Evidence for an Alternative Tricarboxylic Acid Cycle
J. Bacteriol., May 15, 2000; 182(10): 2838 - 2844.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.