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Journal of Experimental Botany, Vol 50, 1627-1628, Copyright © 1999 by Oxford University Press

ARTICLES

Gene note. Cloning of a cDNA encoding the thaumatin-like protein of winter rye (Secale cereale L. Musketeer) and its functional characterization

Y Chan, W Tung, M Griffith and K Chow
Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, ROC; Department of Biology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada; Corresponding author; Fax: +852 2358 1552; E-mail: bckchow@ust.hk

A cDNA clone, WRTLP2, encoding an open reading frame of 173 amino acids, was recovered from a cDNA library of winter rye (Secale cereale L. Musketeer). The amino acid sequence deduced from the cloned cDNA exhibits very high sequence similarity (70-95%) with those of extracellular and low molecular weight thaumatin-like proteins of other cereals. It was possible to overexpress this isolated cDNA in Escherichia coli and it was found that the encoded protein of this clone exhibited antifungal activities against several fungal strains.Keywords: Low molecular weight thaumatin-like protein, antifungal activity, pathogenesis-related protein, winter rye, Secale cereale.
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