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Journal of Experimental Botany, Vol 50, 1763-1768, Copyright © 1999 by Oxford University Press

ARTICLES

Physiological evidence for a proton pump and sodium exclusion mechanisms at the plasma membrane of the marine angiosperm Zostera marina L

J Fernandez, M Garcia-Sanchez and H Felle
Departamento de Biologia Vegetal, Facultad de Ciencias, Universidad de Malaga, Campus de Teatinos s/n, E-29071 Malaga, Spain; Botanisches Institut I, Justus-Liebig-Universitat, Senckenbergstr. 17, D-35390 Giessen, Germany; Corresponding author; Fax: +34 95 2132000; E-mail: ja_fernandez@uma.es

The basic electrical plasma membrane characteristics of leaf cells from the seagrass Zostera marina L. have been investigated with respect to its primary transport system and its Na+/K+ selectivity. In natural seawater Z. marina exhibits a membrane potential of -15610 mV. The phytotoxin fusicoccin stimulates H+ extrusion and hyperpolarizes the plasma membrane. Ouabain, an inhibitor of the mammalian Na+K+-ATPase did not depolarize the plasma membrane of Z.marina. Both flushing the leaves with CO2 and 'light off' acidified the cytoplasm and hyperpolarized the cells. It is suggested that a H+-ATPase rather than a Na+-ATPase is the primary pump in Z.marina. In the presence of cyanide plus salicylhydroxamic acid the membrane potential changed to -6411 mV. This so-called diffusion potential was sensitive to external [K+] from 0.05 to 0.5 mM in the presence of 0.5 M Na+ and revealed a relative permeability PK+/PNa+ of 303. We suggest that this high ratio is the basic adaptation which permits Z. marina to grow in high [Na+] conditions and to exhibit a rather negative resting potential. Since amiloride, an inhibitor of the nH+/Na+ antiporter, hyperpolarized the plasma membrane, it is suggested that this transporter could be present in the plasma membrane of Z. marina acting as an overflow valve for Na+ which leaks into the cell.Keywords: H+-ATPase, cytoplasmic pH, membrane potential, sodium permeability, Zostera
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