Journal of Experimental Botany, Vol. 51, No. 342, pp. 107-113,
January 2000
© 2000 Oxford University Press
Low temperature-induced changes in the distribution of H2O2 and antioxidants between the bundle sheath and mesophyll cells of maize leaves
1 Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, Herts AL5 2JQ, UK
2 Department of Applied Genetics, John Innes Centre, Colney, Norwich NR4 7UH, UK
The distribution of antioxidants between bundle sheath and mesophyll cells of maize leaves was analysed in plants grown at 20 °C, 18 °C and 15 °C. The purity of the isolated bundle sheath and mesophyll fractions was determined using compartment-specific marker enzymes. In plants grown at 15 °C, ascorbate peroxidase, CuZn-superoxide dismutase (CuZn-SOD) and monodehydroascorbate reductase activities were increased in the bundle sheath cells, and glutathione reductase, dehydroascorbate reductase and monodehydroascorbate reductase activities were enhanced in the mesophyll cells. SOD was absent from the mesophyll of plants grown at 20 °C but an Fe-SOD activity was found in the mesophyll of plants grown at 15 °C. Foliar Mn-SOD activities were decreased at 15 °C compared to 20 °C. Catalase was undetectable in the mesophyll extracts of plants grown at 15 °C. Ascorbate and glutathione contents were considerably higher in the mesophyll than the bundle sheath fractions of plants grown at 20 °C. The ratios of reduced to oxidized forms of these antioxidants were significantly decreased in the bundle sheath, but increased in the mesophyll of leaves grown at 15 °C. Foliar H2O2 accumulated at 15 °C compared to 20 °C. Most of the foliar H2O2 was localized in the mesophyll tissues at all growth temperatures. The differential distribution of antioxidants between leaf bundle sheath and mesophyll tissues, observed at 20 °C, is even more pronounced when plants are grown at 15 °C and may contribute to the extreme sensitivity of maize to low temperatures.
Key words: antioxidants. maize, bundle sheath, mesophyll, temperature.
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