Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Sheveleva, E. V.
Right arrow Articles by Bohnert, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sheveleva, E. V.
Right arrow Articles by Bohnert, H. J.
Agricola
Right arrow Articles by Sheveleva, E. V.
Right arrow Articles by Bohnert, H. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 51, No. 342, pp. 115-122, January 2000
© 2000 Oxford University Press

Disturbance in the allocation of carbohydrates to regenerative organs in transgenic Nicotiana tabacum L.

Elena V. Sheveleva1, Richard G. Jensen1,2 and Hans J. Bohnert1,2,3,4

1 Department of Biochemistry, The University of Arizona, Biosciences West, 1041 E. Lowell St, Tucson, Arizona, 85721-0088 USA
2 Department of Plant Sciences, The University of Arizona, Biosciences West, 1041 E. Lowell St, Tucson, Arizona, 85721-0088 USA
3 Department of Molecular and Cellular Biology, The University of Arizona, Biosciences West, 1041 E. Lowell St, Tucson, Arizona, 85721-0088 USA

Transgenic tobacco (Nicotiana tabacum L, cv. SR-1) expressing mannitol 1-phosphate dehydrogenase, MTLD, in chloroplasts and myo-inositol O-methyltransferase, IMT1, in the cytosol after crossing of lines which expressed these foreign genes separately has been analysed. Plants expressing both enzymes accumulated mannitol and -ononitol in amounts comparable to those following single gene transfer and showed phenotypically normal growth during the vegetative stage. Induction of flowering for transgenovar and wild-type occurred at the same time, but during flowering the phenotype of the transformed plants changed. Compared to wild-type, transgenic plants were characterized by curled, smaller upper leaves and elongated stems during flowering; incomplete development of flower buds with shorter sepals and pedicels resulted in increased abortion. Flowers completing development were normal. The vegetative biomass of the transformed plants was slightly higher than that of wild-type. Concentrations of soluble sugars and potassium were lower than in wild-type only in the apical parts of the transgenic plants. Both enzymes, under control of the CaMV 35S promoter, promoted accumulation of mannitol and -ononitol in the youngest leaves close to the vegetative meristem and in flowers, suggesting that their presence could signal lower sink demand leading to a decrease in carbon import to flowers and developing seed capsules. The interpretation here is that increases of inert carbohydrates in developing sinks interfere with metabolism, such as respiration or glycolysis. This interference may be less significant in source tissues during vegetative growth than in sink tissues during seed development.

Key words: transgenic tobacco, mannitol, D-ononitol, source–sink disturbance, seed production.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. I. Zanor, S. Osorio, A. Nunes-Nesi, F. Carrari, M. Lohse, B. Usadel, C. Kuhn, W. Bleiss, P. Giavalisco, L. Willmitzer, et al.
RNA Interference of LIN5 in Tomato Confirms Its Role in Controlling Brix Content, Uncovers the Influence of Sugars on the Levels of Fruit Hormones, and Demonstrates the Importance of Sucrose Cleavage for Normal Fruit Development and Fertility
Plant Physiology, July 1, 2009; 150(3): 1204 - 1218.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Simon-Nobbe, U. Denk, P. B. Schneider, C. Radauer, M. Teige, R. Crameri, T. Hawranek, R. Lang, K. Richter, P. Schmid-Grendelmeier, et al.
NADP-dependent Mannitol Dehydrogenase, a Major Allergen of Cladosporium herbarum
J. Biol. Chem., June 16, 2006; 281(24): 16354 - 16360.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Abebe, A. C. Guenzi, B. Martin, and J. C. Cushman
Tolerance of Mannitol-Accumulating Transgenic Wheat to Water Stress and Salinity
Plant Physiology, April 1, 2003; 131(4): 1748 - 1755.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.